PERFECT FIFTHS VERSUS AN EQUAL TEMPERED KEYBOARD

Because a keyboard is tuned in equal-tempered intervals, where each minor second is equal to the twelfth root of two, a so-called perfect fifth on a keyboard is actually 2 to the (7/12) power, a bit smaller than a perfect fifth that is a ratio of 3:2.  So, if you tune your instrument's A to a keyboard and then tune in perfect fifths, your notes that are lower in the circle of fifths, like D, G and C, will be too low for the corresponding notes on the keyboard.

To 'temper' your tuning, you can play a chord on the keyboard that contains notes from various points around the circle of fifths, and then compare each of your strings to see if they are a close enough fit.  My old violin prof liked a chord made of B, D, F and A.

Instrument Tuning and Note Location